In addition FlowJo has a number of other advanced capabilities, including software compensation and cell-cycle and kinetics analysis platforms. These two features alone make FlowJo worth the price to us. Furthermore, statistics from a large number of samples can be quickly generated, collated and exported for further analysis in Excel or other specialized packages. As I read the literature I can often pick the authors who use FlowJo from the quality of their flow cytometric data presentation. Excellent publication quality output can be generated directly in FlowJo or transferred to other applications for further manipulation. On top of the workspace FlowJo adds a comprehensive set of data presentation tools that include all the standard flow cytometric plots, as well as some extremely useful new types, in particular probability contour plots with outliers. When new samples are added to the workspace FlowJo can be setup to automatically match them to pre-existing groups and automatically perform the same analysis and reports. The workspace approach also makes batch analysis extremely quick and simple as samples can be placed into groups and then the same analysis applied to all the samples with a single drag ‘n drop. This means that you have a complete and persistent record of all the analysis you perform, enabling you to easily review what you have done or to leave an analysis and return to it later. We use the current version 3.2 (released July 2000) every day to analyze and present the data from our highly complex flow cytometry-based experiments.įlowJo is based around “workspace” documents that contain the samples from your experiment, along with all of the gates, graphs, statistics, tables and reports that you apply to, or generate from, your data. Three years, several program revisions, many experiments and thousands of megabytes of data later, FlowJo has proven to be an extremely valuable tool.
For some time we had been frustrated with the applications available for analyzing our flow cytometric data and FlowJo promised to overcome many of those frustrations.
When FlowJo 2.1 was first released in June 1997 it generated considerable interest in our laboratory.
0 kommentar(er)
